Fig 1: OIP5-AS1 upregulates CCNG1 expression via sponging miR-128-3p in OC cells. (A) mRNA and (B) protein expression of CCNG1 was measured by reverse transcription-quantitative PCR and western blotting in OVCAR-3 and SKOV3 cells transfected with miR-128-3p, miR-128-3p + OIP5-AS1 or their relative controls. *P<0.05. OIP5-AS1, o-phthalaldehyde-interacting protein 5 antisense transcript 1; CCNG1, cyclin G1; miR, microRNA; OC, ovarian cancer.
Fig 2: OIP5-AS1 depression inhibited tumor growth of OC by miR-128-3p/CCNG1 axis in vivo. (A) Tumor volume and (B) weight were measured in sh-OIP5-AS1 and sh-control groups. (C) The levels of OIP5-AS1 and (D) miR-128-3p were examined using reverse transcription-quantitative PCR. (E) CCNG1 protein expression was measured using western blotting. *P<0.05. OIP5-AS1, o-phthalaldehyde-interacting protein 5 antisense transcript 1; OC, ovarian cancer; miR, microRNA; sh-, short hairpin; CCNG1, cyclin G1.
Fig 3: Downregulation of miR-128-3p restored the si-CCNG1-induced effect on OC cells. (A and B) Cell viability was examined by MTT assay after transfection of si-CCNG1, si-CCNG1+anti-miR-128-3p or the corresponding controls. (C and D) Cell apoptosis was detected by flow cytometry. (E and F) Cell migration and (G and H) invasion were measured by Transwell assay. The examination of (I and J) glucose consumption and (K and L) lactate production was performed via glucose detection and lactic acid detection kits. (M and N) The protein expression of HK2 was determined by western blotting assay. *P<0.05. miR, microRNA; si-, small interfering; CCNG1, cyclin G1; OC, ovarian cancer; HK2, hexokinase 2.
Fig 4: CCNG1 is a target of miR-128-3p. (A) The potential target of miR-128-3p was predicted by TargetScan software. (B) The relationship between miR-128-3p and CCNG1 was explored by the dual-luciferase reporter assay. The mRNA and protein expression levels of CCNG1 were determined by (C) RT-qPCR and (D) western blotting assays in OC cells. (E) The inhibitory effect of anti-miR-128-3p on miR-128-3p level was assayed by RT-qPCR. The determination of CCNG1 was performed by (F) RT-qPCR and (G) western blotting in OVCAR-3 and SKOV3 cells transfected with miR-128-3p, anti-miR-128-3p or the respective controls. *P<0.05. CCNG1, cyclin G1; miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR; WT, wild-type; MUT, mutant-type.
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